Hi @User007, Yes, You can definitely do that:
In the Source Library panel, select your video layer (by clicking on it).
In the Video layer properties, make sure the loop option is disabled and the "Clear when Finished" option is on (green).
Add your video to whichever scene (or scenes) you want (If you already placed the video in your scene you don't have to do it again).
Now, whenever you switch to a scene with that video in it, the video will play once and then
will be cleared from the scene.